GLC is used to separate a wide variety of organic compounds. The more common technique (which will be used in this experiment) is gas-liquid chromatography (GLC) in which the stationary phase is a porous solid covered with an absorbing liquid. This technique is generally used to separate volatile compounds in a gaseous solution. When the stationary phase is a solid, the separation process is more precisely called gas-solid chromatography.
Gas chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one being a stationary bed of large surface area, and the other a gas that percolates through the stationary bed.
The NIST 2005 library, which is a software feature of the GC/MS data acquisition system, will be utilized to confirm the identification of the components of the unknown. Thus, the characteristic features of a mass spectrum for a given class may be recognized, and the chemical structure determined. In this lab, the ITD spectrum you measure of the unknown will be compared to the EI spectra of several different classes of compounds found in the library. These classical spectra are used in the National Bureau of Standards library of mass spectra for comparison differences. Due to the design variances of the ITD compared to a true quadrupole mass spectrometer, the ITD mass spectrum of an organic compound may not be identical (but should be very similar) to its classical mass spectrum measured by an electron impact (EI) detector. The ITD is a variation of a quadrupole mass spectrometer and is designed to function specifically as a GC detector. The analysis of different unknown mixtures will be performed via high resolution capillary gas chromatography (GC) coupled with an ion trap detector (ITD). This lab has an added challenge of first identifying what the contents of those standards are before being drawing conclusions about the mixtures.
235000003373 curcuma longa Nutrition 0.000 title abstract description 10.235000003392 Curcuma domestica Nutrition 0.000 title abstract description 10.238000002360 preparation method Methods 0.000 title claims abstract description 8.239000000126 substance Substances 0.000 title claims abstract description 17.239000000341 volatile oil Substances 0.000 title claims abstract description 18.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Guizhou University filed Critical Guizhou University Priority to CN201410103616.1A priority Critical patent/CN104478686B/en Publication of CN104478686A publication Critical patent/CN104478686A/en Application granted granted Critical Publication of CN104478686B publication Critical patent/CN104478686B/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee Guizhou University Priority date (The priority date is an assumption and is not a legal conclusion. Guangxi Mojiang biopharmaceutical Co., Ltd Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 周英 潘博文 石洋 王慧娟 崔凯 刘雄利 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN201410103616.1A Other languages Chinese ( zh)
Google Patents Preparation method of ar-turmerone reference substance in turmeric volatile oilĭownload PDF Info Publication number CN104478686A CN104478686A CN201410103616.1A CN201410103616A CN104478686A CN 104478686 A CN104478686 A CN 104478686A CN 201410103616 A CN201410103616 A CN 201410103616A CN 104478686 A CN104478686 A CN 104478686A Authority CN China Prior art keywords ethyl acetate substance petroleum ether turmerone volatile oil Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN104478686A - Preparation method of ar-turmerone reference substance in turmeric volatile oil CN104478686A - Preparation method of ar-turmerone reference substance in turmeric volatile oil